Regulatory
Str(e)pA

Part:BBa_K4590001:Design

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


SARP Cosmomycin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 204
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 475
    Illegal XhoI site found at 525
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 204
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 204
    Illegal NgoMIV site found at 661
    Illegal AgeI site found at 140
    Illegal AgeI site found at 343
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 683


Design Notes

The sequence was not properly identified. To work with it, we identified its conserved domains and its proximity to the cosmomycin cluster.


Source

Streptomyces olindensis strain DAUFPE 5622 2, whole genome shotgun sequence. GenBank: JJOH01000002.1

References